دانلود Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies

ترجمه فارسی Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies
قیمت : 315,000 ریال
شناسه محصول : 2009206
نویسنده/ناشر/نام مجله : Gene regulation and systems biology
سال انتشار: 2016
تعداد صفحات انگليسي : 9
نوع فایل های ضمیمه : pdf+word
حجم فایل : 608 Kb
کلمه عبور همه فایلها : www.daneshgahi.com
عنوان انگليسي : Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies

چکیده

Abstract

To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated  ChIP  (enChIP)  using  the  clustered  regularly  interspaced  short  palindromic  repeats  (CRISPR)  system  or  transcription  activator-like) TAL) proteins. Essentially, a locus-specific ChIP consists of locus-tagging and affinity purification and can be combined with downstream analyses to identify molecules associated with the target genomic regions. In this review, we discuss the applications of locus-specific ChIP to analyze the genome functions, including transcription and epigenetic regulation.

Keywords: ChIP chromatin immunoprecipitation locus-specific ChIP iChIP
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