دانلود Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies

Abstract

To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated  ChIP  (enChIP)  using  the  clustered  regularly  interspaced  short  palindromic  repeats  (CRISPR)  system  or  transcription  activator-like) TAL) proteins. Essentially, a locus-specific ChIP consists of locus-tagging and affinity purification and can be combined with downstream analyses to identify molecules associated with the target genomic regions. In this review, we discuss the applications of locus-specific ChIP to analyze the genome functions, including transcription and epigenetic regulation.