دانلود In Vitro Culture of Zygotic Embryos of Ilex Species

ترجمه مقاله In Vitro Culture of Zygotic Embryos of Ilex Species
قیمت : 195,000 ریال
شناسه محصول : 2009649
نویسنده/ناشر/نام مجله : HortScience
سال انتشار: 2001
تعداد صفحات انگليسي : 2
نوع فایل های ضمیمه : Pdf+Word
حجم فایل : 2 Mb
کلمه عبور همه فایلها : www.daneshgahi.com
عنوان انگليسي : In Vitro Culture of Zygotic Embryos of Ilex Species

چکیده

 Abstract

Plants of Ilex argentina L., I. brasiliensis (S.) L., I. brevicuspis R., I. dumosa R., I. integerrima (V.C.) L., I. microdonta R., I. pseudoboxus R., and I. theezans C.M. were obtained by immature embryo culture. Heart-stage zygotic embryos were removed from immature fruits and cultured aseptically on quarter-strength Murashige and Skoog medium with 3% sucrose, 0.65% agar, and 0.1 mg·L-1 zeatin. Cultures were incubated at 27±2°C for 4 weeks, in darkness and subsequently transferred to a culture room with a 14-hour photoperiod (116 μmol·m-2·s-1) for another 4 weeks. Seedlings with two leaves, derived from germinated embryos, were successfully transplanted to pots containing 1 peat: 1 perlite: 1 sand (v/v) and were maintained in greenhouse conditions. From 95% to 100% of transplanted seedlings survived. Chemical name used: 6-(4-hydroxy-3-methylbut-2-enylamino) purine (zeatin).

 

 

Keywords: Zygotic Embryos In Vitro Culture
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