دانلود A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies

ترجمه A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies
قیمت : 285,000 ریال
شناسه محصول : 2009204
نویسنده/ناشر/نام مجله : F1000Research
سال انتشار: 2015
تعداد صفحات انگليسي : 10
نوع فایل های ضمیمه : pdf+word
حجم فایل : 646 Kb
کلمه عبور همه فایلها : www.daneshgahi.com
عنوان انگليسي : A ChIP on the shoulder? Chromatin immunoprecipitation and validation strategies for ChIP antibodies

چکیده

Abstract

Chromatin immunoprecipitation (ChIP) is a technique widely used in the study of epigenetics and transcriptional regulation of gene expression. However, its antibody-centric nature exposes it to similar challenges faced by other antibody-based procedures, of which the most prominent are issues of specificity and affinity in antigen recognition. As with other techniques that make use of antibodies, recent studies have shown the need for validation of ChIP antibodies in order to be sure they recognize the advertised protein or epitope. We summarize here the issues surrounding ChIP antibody usage, and highlight the toolkit of validation methods that can be employed by investigators looking to appraise these reagents.

Keywords: Chromatin immunoprecipitation antibody validation transcription fa
این برای گرایش های: کلیه گرایش ها، کاربرد دارد. [ برچسب: ]

Skip Navigation Links